Newly designed primer sets available for evaluating various cytokines and iNOS mRNA expression in guinea pig lung tissues by RT-PCR.

@article{Yamada2005NewlyDP,
  title={Newly designed primer sets available for evaluating various cytokines and iNOS mRNA expression in guinea pig lung tissues by RT-PCR.},
  author={Hiroyuki Yamada and Tadashi Udagawa and Satoru Mizuno and Kumiko Hiramatsu and Isamu Sugawara},
  journal={Experimental animals},
  year={2005},
  volume={54 2},
  pages={163-72}
}
Guinea pigs are often used as an animal model of human tuberculosis (TB). However, there are few methods available for pursuing the immunological processes involved in guinea pig TB. In this study, we developed for the first time systematic reverse transcription (RT)-PCR for evaluation of guinea pig mRNA expression. RT-PCR primer sets were newly designed for detection of cytokines and inducible nitric oxide synthase (iNOS) mRNA in guinea pig TB. Interferon (IFN)-gamma, tumor necrosis factor… CONTINUE READING
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