New wound-healing model using cultured corneal endothelial cells. 1. Quantitative study of healing process.

@article{Miyata1990NewWM,
  title={New wound-healing model using cultured corneal endothelial cells. 1. Quantitative study of healing process.},
  author={Keisuke Miyata and M. Murao and Mitsuru Sawa and Takeo Tanishima},
  journal={Japanese journal of ophthalmology},
  year={1990},
  volume={34 3},
  pages={257-66}
}
A new in vitro model to evaluate the corneal endothelial wound-healing process was developed. Confluent monolayer cell sheets of either rabbit or bovine corneal endothelial cells were cultured on cover slips, the surface of which had been precoated with hydroxyethyl methacrylate (HEMA) except for the 8 mm-diameter area for cell culture. Wounds were made by scraping off cells in the center of the cell sheets using a rotating silicone tip. The areas of these wounds were measured by a method of… CONTINUE READING

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