New topoisomerase essential for chromosome segregation in E. coli.

@article{Kato1990NewTE,
  title={New topoisomerase essential for chromosome segregation in E. coli.},
  author={Jun-ichi Kato and Yukinobu Nishimura and Ryu Imamura and Hironori Niki and Sota Hiraga and Hiroyuki Suzuki},
  journal={Cell},
  year={1990},
  volume={63 2},
  pages={393-404}
}
The nucleotide sequence of the parC gene essential for chromosome partition in E. coli was determined. The deduced amino acid sequence was homologous to that of the A subunit of gyrase. We found another new gene coding for about 70 kd protein. The gene was sequenced, and the deduced amino acid sequence revealed that the gene product was homologous to the gyrase B subunit. Mutants of this gene were isolated and showed the typical Par phenotype at nonpermissive temperature; thus the gene was… CONTINUE READING