New tools for the generation of E1- and/or E3-substituted adenoviral vectors

@article{Danthinne2000NewTF,
  title={New tools for the generation of E1- and/or E3-substituted adenoviral vectors},
  author={Xavier Danthinne and Esther Werth},
  journal={Gene Therapy},
  year={2000},
  volume={7},
  pages={80-87}
}
We have designed new vectors for the construction of recombinant adenoviruses containing expression cassettes in the E1 and/or E3 regions. Using a versatile set of restriction enzymes, the cassettes are cloned into small bacterial vectors and subsequently introduced into large plasmids containing the adenoviral sequences. Two positive selection markers facilitate the recovery of a cosmid containing a copy of the sequence of the recombinant adenovirus. The resulting cosmid is transfected into… CONTINUE READING