New tools for phenotypic analysis in Candida albicans: the WAR1 gene confers resistance to sorbate.

@article{Lebel2006NewTF,
  title={New tools for phenotypic analysis in Candida albicans: the WAR1 gene confers resistance to sorbate.},
  author={Karine Lebel and Sarah MacPherson and Bernard Turcotte},
  journal={Yeast},
  year={2006},
  volume={23 4},
  pages={
          249-59
        }
}
Availability of the complete sequence of the Candida albicans genome allows for global gene analysis. We designed a gene deletion method to facilitate such studies. First, we constructed C. albicans strains that are both Deltaura3 and Deltatrp1. Second, we designed a system that relies on in vitro recombination, using the Gateway((R)) technology, for efficient generation of deletion cassettes. They are generated in two steps: (a) upstream and downstream DNA fragments of the chromosomal region… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-9 OF 9 CITATIONS

A TRP5/5-fluoroanthranilic acid counter-selection system for gene disruption in Candida guilliermondii

VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Genetic surgery in fungi: employing site-specific recombinases for genome manipulation

  • Sven Krappmann
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • 2013
VIEW 2 EXCERPTS
CITES METHODS