New tools for labeling silica in living diatoms.

@article{Descls2008NewTF,
  title={New tools for labeling silica in living diatoms.},
  author={Julien Descl{\'e}s and Mathieu Vartanian and Abdeslam El Harrak and Michelle Quinet and Nicolas Br{\'e}mond and Guillaume Sapriel and J'erome Bibette and Pascal Jean Lopez},
  journal={The New phytologist},
  year={2008},
  volume={177 3},
  pages={
          822-9
        }
}
Silicon biomineralization is a widespread mechanism found in several kingdoms that concerns both unicellular and multicellular organisms. As a result of genomic and molecular tools, diatoms have emerged as a good model for biomineralization studies and have provided most of the current knowledge on this process. However, the number of techniques available to study its dynamics at the cellular level is still rather limited. Here, new probes were developed specifically to label the pre-existing… 

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References

SHOWING 1-10 OF 41 REFERENCES
A new molecular tool for transgenic diatoms
TLDR
It is demonstrated that the Pnr/Tnr cassette enables inducible gene expression and control of both the level and timing of mRNA and protein expression in transgenic diatoms.
Diatomics: toward diatom functional genomics.
TLDR
The different techniques available to investigate gene and protein function in diatoms are reviewed and the first large-scale analysis of 12000 expressed sequence tags is accomplished and organized in a queryable database, Phaeodactylum tricornutum database (PtDB).
Silica transport in the demosponge Suberites domuncula: fluorescence emission analysis using the PDMPO probe and cloning of a potential transporter.
TLDR
Results from combined cytobiological and molecularbiological techniques suggest that, in the demosponge Suberites domuncula, silicic acid is taken up by a transporter that is involved in silica uptake and is termed the NBCSA [Na+/HCO3-[Si(OH)4]] co-transporter.
Silicon in the photosynthetic lineages: molecular mechanisms for uptake and deposition.
TLDR
The great potentiality of using Si biomineralization processes has recently been realized, for both plant biotechnology purposes and for the development of new biomimetic or “eco-friendly” approaches in Si chemistry and new materials design.
Identification of Proteins from a Cell Wall Fraction of the Diatom Thalassiosira pseudonana
TLDR
This is the first proteomic analysis of a diatom, and furthermore, new candidate genes involved in structure formation are identified and the involvement of one enzyme (and its gene) in the structure formation process is directly demonstrated.
Characterizing the silica deposition vesicle of diatoms
SummaryThe present study on a centric diatom,Ditylum brightwellii, includes two parts: detection of sugars in the silica deposition vesicle (SDV) with lectins and labeling the developing siliceous
Prospects in diatom research.
SILICON DEPOSITION IN DIATOMS: CONTROL BY THE pH INSIDE THE SILICON DEPOSITION VESICLE
TLDR
The results with DAMP suggest that the pH of the SDV is important in the silicification of diatoms: It facilitates a fast nucleation and aggregation of silica particles, thus increasing the rate of formation of the mature frustules.
Controlled silica synthesis inspired by diatom silicon biomineralization.
TLDR
The synthesis of tailor-made mesoporous silicas in which the author can control the morphological features of the resulting materials on the nanometer level as well as on the micrometer level is demonstrated.
...
1
2
3
4
5
...