New tissue dissociation protocol for scaled-up production of neural stem cells in suspension bioreactors.

@article{Sen2004NewTD,
  title={New tissue dissociation protocol for scaled-up production of neural stem cells in suspension bioreactors.},
  author={Arindom Sen and Michael S Kallos and Leo A. Behie},
  journal={Tissue engineering},
  year={2004},
  volume={10 5-6},
  pages={
          904-13
        }
}
The successful dissociation of mammalian neural stem cell (NSC) aggregates (neurospheres) into a single-cell suspension is an important procedure when expanding NSCs for clinical use, or when performing important assays such as clonal analyses. Until now, researchers have had to rely primarily on destructive mechanical methods such as trituration with a pipette tip to break apart the aggregates. In this study we report on a new chemical dissociation procedure that is efficient, cost effective… CONTINUE READING
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