New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities.

@article{Osborne2006NewTA,
  title={New threshold and confidence estimates for terminal restriction fragment length polymorphism analysis of complex bacterial communities.},
  author={Catherine A Osborne and Gavin N. Rees and Yaniv Bernstein and Peter H. Janssen},
  journal={Applied and environmental microbiology},
  year={2006},
  volume={72 2},
  pages={1270-8}
}
Terminal restriction fragment length polymorphism (T-RFLP) analysis has the potential to be useful for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables. To do this successfully, systematic variations have to be detected above method-associated noise, by standardizing data sets and assigning confidence estimates to relationships detected. We investigated the use of different standardizing methods in T-RFLP… CONTINUE READING
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