New thermostable amylase from Bacillus cohnii US147 with a broad pH applicability.

@article{Ghorbel2009NewTA,
  title={New thermostable amylase from Bacillus cohnii US147 with a broad pH applicability.},
  author={Raoudha Ellouz Ghorbel and Sameh Maktouf and Ezedine Ben Massoud and Samir Bejar and Semia Ellouz Chaabouni},
  journal={Applied biochemistry and biotechnology},
  year={2009},
  volume={157 1},
  pages={50-60}
}
A new thermophilic bacterial strain identified as Bacillus cohnii US147 was isolated from the southern Tunisian soil. The identification was based on physiological tests and molecular techniques related to the 16S ribosomal ribonucleic acid. The isolated strain produced amylase, which was purified. This amylase had an apparent molecular mass of 30 kDa as estimated by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Amylase US147 showed K (m) and V (max) values of 0.7 mg/ml and 2… CONTINUE READING