New technique for distinguishing between human chromosomes.

@article{Sumner1971NewTF,
  title={New technique for distinguishing between human chromosomes.},
  author={A. T. Sumner and H. Evans and R. Buckland},
  journal={Nature: New biology},
  year={1971},
  volume={232 27},
  pages={
          31-2
        }
}
A GIEMSA staining procedure that preferentially stains centromeric heterochromatin in mouse chromosomes has been described1. This specificity was observed when fixed preparations were treated with sodium hydroxide to denature the DNA, and then incubated in warm saline to allow annealing, in the presence of 3H-labelled single stranded satellite DNA or its complementary RNA. In this way mouse satellite DNA was located in the centromeric heterochromatin1,2. It is known to consist of highly… Expand
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