New strategy for selective and sensitive assay of cathepsin B using a dityrosine-based material.

@article{Kim2013NewSF,
  title={New strategy for selective and sensitive assay of cathepsin B using a dityrosine-based material.},
  author={Chan-Jin Kim and Dong-Ik Lee and Dong Zhang and C. Lee and I. Ahn},
  journal={Analytical biochemistry},
  year={2013},
  volume={435 2},
  pages={
          166-73
        }
}
The increasing number of reports for disease-related proteases has necessitated materials for the fast, sensitive, and specific assessment of protease activities. The purpose of this study was to synthesize and test a dityrosine-based substrate for the selective assay of a specific cysteine cathepsin. DBDY-Gly-INH)2 was synthesized from the conjugation of N,N'-diBoc-dityrosine (DBDY) with two molecules of glycine and isoniazid (INH) for this purpose. The fluorescence of DBDY (λex=284-320nm, λem… Expand
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