New strategies for cultivation and detection of previously uncultured microbes.

@article{Stevenson2004NewSF,
  title={New strategies for cultivation and detection of previously uncultured microbes.},
  author={Bradley S Stevenson and Stephanie A Eichorst and John T Wertz and Thomas M. Schmidt and John A. Breznak},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 8},
  pages={4748-55}
}
An integrative approach was used to obtain pure cultures of previously uncultivated members of the divisions Acidobacteria and Verrucomicrobia from agricultural soil and from the guts of wood-feeding termites. Some elements of the cultivation procedure included the following: the use of agar media with little or no added nutrients; relatively long periods of incubation (more than 30 days); protection of cells from exogenous peroxides; and inclusion of humic acids or a humic acid analogue… CONTINUE READING
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