New sandwich-type enzyme-linked immunosorbent assay for human MxA protein in a whole blood using monoclonal antibodies against GTP-binding domain for recognition of viral infection.

@article{Kawamura2012NewSE,
  title={New sandwich-type enzyme-linked immunosorbent assay for human MxA protein in a whole blood using monoclonal antibodies against GTP-binding domain for recognition of viral infection.},
  author={Mizuho Kawamura and Akira Kusano and Akiko Furuya and Nobuo Hanai and Hideki Tanigaki and Akihito Tomita and Akira Horiguchi and Kyosuke Nagata and Toshiko Itazawa and Yuichi Adachi and Yoshie Okabe and Toshio Miyawaki and Hiroaki Kohno},
  journal={Journal of clinical laboratory analysis},
  year={2012},
  volume={26 3},
  pages={174-83}
}
OBJECTIVES To develop a clinically significant and practical enzyme-linked immunosorbent assay (ELISA) for the detection of MxA protein in human whole blood, a biological marker of viral infection. DESIGN AND METHODS A sandwich ELISA suitable for the measurement of human MxA protein in whole blood was developed using mouse monoclonal antibodies (mAbs) raised against the GTP-binding domain of human MxA protein. Prior to the assay, the whole blood sample was treated with special buffer to… CONTINUE READING