New reporter cell lines to study macrophage-tropic HIV envelope protein-mediated cell-cell fusion.

@article{Hong1999NewRC,
  title={New reporter cell lines to study macrophage-tropic HIV envelope protein-mediated cell-cell fusion.},
  author={Yude Hong and Lily Wu and Min Yi Cui and G. M. Luke McMaster and Stephen W. Hunt and F Z Chung},
  journal={AIDS research and human retroviruses},
  year={1999},
  volume={15 18},
  pages={1667-72}
}
The infection of human cells by HIV-1 virus can be mimicked by a fusion process between cells expressing the HIV envelope protein (Env) and cells expressing both human CD4 (huCD4) and appropriate human chemokine receptors. In this study, a macrophage-tropic (M-tropic) HIV cell-cell fusion assay was established that utilized huCD4, human CCR5 (huCCR5), and HIV ADAgpl60 as fusion components and a Gal4/VP16-activated luciferase as a reporter system. By combining CHO cells expressing huCD4 and… CONTINUE READING

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