New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples.

@article{Gueimonde2004NewRQ,
  title={New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples.},
  author={Miguel Gueimonde and Satu T{\"o}lkk{\"o} and Teemu Korpim{\"a}ki and Seppo Salminen},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 7},
  pages={4165-9}
}
The application of a real-time quantitative PCR method (5' nuclease assay), based on the use of a probe labeled at its 5' end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities of the primers and the primer-probe combination were evaluated by conventional PCR and real-time PCR, respectively. The results obtained by real-time PCR were compared with those obtained by fluorescent in situ hybridization, the current… CONTINUE READING
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