New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities.

@article{Gee2000NewRF,
  title={New ratiometric fluorescent calcium indicators with moderately attenuated binding affinities.},
  author={Kyle R. Gee and Eric A Archer and L A Lapham and Mary E Leonard and Z L Zhou and J Bingham and Zhenjun Diwu},
  journal={Bioorganic & medicinal chemistry letters},
  year={2000},
  volume={10 14},
  pages={1515-8}
}
Mono-halogenated derivatives of the calcium indicators fura-2 and indo-1 were synthesized and their spectroscopic properties evaluated. Halogenation ortho or para to the bridging oxygen in the BAPTA nucleus had a more pronounced weakening effect on binding affinity than in the meta position in the fura derivatives. Two new excitation ratioable fluorescent calcium indicators, benzothiaza-1 and 2, were also synthesized. Kd values of 400 nM to 5.3 microM [Ca2+] were observed in these families of… CONTINUE READING

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