New quinones and hydroquinones from Malbranchea cinnamomea HKI 286 and HKI 296 and interaction with Tax/CREB expression system in yeast.

  title={New quinones and hydroquinones from Malbranchea cinnamomea HKI 286 and HKI 296 and interaction with Tax/CREB expression system in yeast.},
  author={Brigitte Schlegel and Frank H{\"a}nel and Friedrich A. Gollmick and Hans Peter Saluz and Udo Gr{\"a}fe},
  journal={The Journal of antibiotics},
  volume={56 11},
In addition to malbranicin (1) and dihydromalbranicin (5), new substituted quinones 2, 3, 6 and hydroquinone 4 were isolated from the culture brothes of two strains of Malbranchea cinnamomea. The chemical constitutions of new metabolites 2, 3, 4 and 6 were elucidated by optical spectroscopy, mass spectrometry and 1D/2D NMR spectroscopy. 2 (7-methoxymalbranicin) at a concentration of 42 microM inhibited by 67% Tax/CREB-mediated expression of beta-galactosidase in a recombinant strain of… 
8 Citations

Figures and Tables from this paper

α-Glucosidase Inhibitors from Malbranchea flavorosea.
From an extract prepared from the grain-based culture of Malbranchea flavorosea two new polyketides were isolated, namely, 8-chloroxylarinol A and flavoroseoside, and the X-ray structure of compound 6 is reported for the first time.
Molecular Analysis of a 4-Dimethylallyltryptophan Synthase from Malbranchea aurantiaca*
This study provides a detailed characterization of a prenyltransferase in Malbranchea species, reveals two enzyme inhibitors, and through site-directed mutagenesis identified several key amino acid residues in catalysis, yielding new insights into this important yet understudied class of natural product biosynthetic enzymes.
Capnellenes from the Soft Coral Dendronephthya rubeola
Four new acetoxycapnellenes 1–4, the first epoxyprecapnellene 5, along with the known dihydroxycapnellene 6 and monoacetoxycapnellene 7, have been isolated from the soft coral Dendronephthya rubeola.
α-Glucosidase and PTP-1B Inhibitors from Malbranchea dendritica
Docking analysis with yeast α-glucosidase and the C-terminal subunit of human maltase-glucoamylase predicted that 1, 1a, and 1b bind to an allosteric site of the enzymes, which revealed that 1 is a mixed-type inhibitor.
Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15.
While the new analogues were found to be inactive in cancer cell cytotoxicity and antimicrobial assays, they may offer new insights in the context of nontoxic ansamycin-based Hsp90 inhibitors for the treatment of neurodegenerative disease.
Current awareness on yeast
  • Biology
  • 2004
In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts.


A novel quinone antibiotic from Malbranchea cinnamomea TAIM 13T54.
A novel quinone antibiotic named malbranicin was isolated from the culture filtrate and mycelium of Malbranchea cinnamomea TAIM 13T54, a thermophilic fungus and exhibited antimicrobial and cytotoxic activities against Gram-positive bacteria and mammalian cell lines, respectively.
The oncoprotein Tax of the human T-cell leukemia virus type 1 activates transcription via interaction with cellular ATF-1/CREB factors in Saccharomyces cerevisiae
It is demonstrated that the Tax transactivation assay using S. cerevisiae can be successfully applied to identify other cellular proteins forming ternary complexes with Tax and 21-bp repeats in vivo and is critically dependent on the presence of cellular CREB or ATF-1 in vivo.
An alternative pathway for gene regulation by Myc
The data suggest a model for how gene repression by Myc may occur in vivo and identify a novel POZ domain Zn finger protein (Miz‐1) that specifically interacts with Myc, but not with Max or USF.
Regulatory elements involved in tax-mediated transactivation of the HTLV-I LTR.
The results suggest that tax activates gene expression by either direct or indirect interaction with several cellular transcription factors that bind to the HTLV-I LTR.
Inhibition of replication of the etiologic agent of acquired immune deficiency syndrome (human T-lymphotropic retrovirus/lymphadenopathy-associated virus) by avarol and avarone.
Both avarone and avarol block in a dose-dependent manner the expression of the p24 and p17 gag proteins of HTLV-III in H9 cells after virus infection and block viral replication, as judged by approximately 80% inhibition of reverse transcriptase activity.
Human T-cell lymphotropic virus type I (HTLV-I) transcriptional activator, Tax, enhances CREB binding to HTLV-I 21-base-pair repeats by protein-protein interaction.
  • L. Zhao, C. Giam
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
One mechanism by which Tax activates transcription may be mediated through the direct interaction with CREB homodimer and/or CREB/ATF-1 heterodimer to stabilize their assembly on the Tax-responsive CRE motifs in the HTLV-I enhancer.
Molecular mechanism of cell cycle progression induced by the oncogene product Tax of human T-cell leukemia virus type I
The results indicate the important role of Tax-mediated trans-activation of the genes for cell cycle regulatory molecules in Tax-induced cell cycle progression.
Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma
The number of these particle-associated proteins is consistent with the expected proteins of a retrovirus, but the sizes of some are distinct from those of most known retroviruses of the primate subgroups.
Antibodies to adult t‐cell leukemia‐virus‐associated antigen (atla) in sera from patients with atl and controls in japan: A nation‐wide sero‐epidemiologic study
Examination of sera from healthy donors aged 6 to 80 years in ATL‐endemic areas showed an age‐dependent increase of seropositive donors with a maximum of about 30% at 40 years of age, which was higher than that of healthy donors of patients with ATL.