New properties of inclusion bodies with implications for biotechnology.

  title={New properties of inclusion bodies with implications for biotechnology.},
  author={{\vS}pela Peternel and Simona Jev{\vs}evar and Marjan Bele and Vladka Gaberc-Porekar and Viktor Menart},
  journal={Biotechnology and applied biochemistry},
  volume={49 Pt 4},
Human G-CSF (granulocyte colony-stimulating factor) is a well-known biopharmaceutical drug being mostly produced by overexpression in Escherichia coli, where it appears in the form of IBs (inclusion bodies). Following our initial findings that properties of inclusion bodies strongly depend on the growth conditions used, especially growth temperature, we compared the characteristics of the G-CSF inclusion bodies prepared at two different temperatures, namely 42 and 25 degrees C. IBs formed at… CONTINUE READING


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