New procedure for detecting antinuclear antibodies using glucose oxidase immunoenzyme technic.

Abstract

Enzyme glucose oxidase was used as the label on the secondary antibody in an indirect ANA-test procedure using rat kidney tissue as the antigen-source. Glucose oxidase is not endogenously present in mammalian tissues and, therefore, produces no background staining as obtained with flurochrome and peroxidase labels, which are commonly used to detect tissue… (More)

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