New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians

  title={New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians},
  author={Catarina Pinho and Sara M Rocha and Bruno Maia Carvalho and Susana H Lopes and Sofia Mour{\~a}o and Marcelo Vallinoto and Tuliana O. Brunes and C{\'e}lio Fernando Baptista Haddad and Helena R. Gonçalves and Fernando Sequeira and Nuno Ferrand},
  journal={Conservation Genetics Resources},
We report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura… CONTINUE READING
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