New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins.

@article{Mohamed2003NewPF,
  title={New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins.},
  author={S A Mohamed and Tove M. I. E. Christensen and Jorn Dalgaard Mikkelsen},
  journal={Carbohydrate research},
  year={2003},
  volume={338 6},
  pages={515-24}
}
Two extracellular isoenzymes of polygalacturonases PG1 and PG2 were isolated from 3-day-old culture filtrates of Trichoderma reesei. The two enzymes were purified to homogeneity by ion-exchange, gel filtration and hydrophobic interaction chromatographies. PG1 and PG2 exhibit similar molecular weights from gel filtration and SDS-PAGE. Their properties, including optimal pH and temperature, thermal stability and Km were compared. Characterization of substrate specificity showed that the two… CONTINUE READING
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