New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli.

@article{Rimm1989NewPV,
  title={New plasmid vectors for high level synthesis of eukaryotic fusion proteins in Escherichia coli.},
  author={David L. Rimm and Thomas D. Pollard},
  journal={Gene},
  year={1989},
  volume={75 2},
  pages={323-7}
}
Production of eukaryotic proteins in Escherichia coli has become rather simple since commercially available bacteriophage and plasmid vector systems allow investigators to select the optimal system for their particular problem. A common question is which system to use to produce the largest quantity of soluble recombinant protein with minimal, if any, bacterial protein fused to it. We have constructed a new set of plasmid vectors that produce large amounts of a fusion proteins that contain less… CONTINUE READING

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