New photocycle intermediates in the photoactive yellow protein from Ectothiorhodospira halophila: picosecond transient absorption spectroscopy.

@article{Ujj1998NewPI,
  title={New photocycle intermediates in the photoactive yellow protein from Ectothiorhodospira halophila: picosecond transient absorption spectroscopy.},
  author={L. Ujj and Savitha Devanathan and Terry E. Meyer and Michael A. Cusanovich and Gordon Tollin and Geoffrey Atkinson},
  journal={Biophysical journal},
  year={1998},
  volume={75 1},
  pages={406-12}
}
Previous studies have shown that the room temperature photocycle of the photoactive yellow protein (PYP) from Ectothiorhodospira halophila involves at least two intermediate species: I1, which forms in <10 ns and decays with a 200-micros lifetime to I2, which itself subsequently returns to the ground state with a 140-ms time constant at pH 7 (Genick et al. 1997. Biochemistry. 36:8-14). Picosecond transient absorption spectroscopy has been used here to reveal a photophysical relaxation process… CONTINUE READING