New pUC-derived expression vectors for rapid construction of cDNA libraries.

@article{Oberbumer1986NewPE,
  title={New pUC-derived expression vectors for rapid construction of cDNA libraries.},
  author={Ilse Oberb{\"a}umer},
  journal={Gene},
  year={1986},
  volume={49 1},
  pages={81-91}
}
We have constructed a new series of the pUC-derived plasmids with an extended polylinker obtained from M13tg131 [Kieny et al., Gene 26 (1983) 91-99]. These vectors allowed us to design a simplified version of the method of Okayama and Berg [Mol. Cell. Biol. 2 (1982) 161-170] for establishing complete cDNA libraries. Improvements included easy recovery of the inserted cDNA due to the extended polylinkers; use of these vectors for gene expression in Escherichia coli, and amenability to supercoil… CONTINUE READING