New oligonucleotide primers for P-typing of rotavirus strains: Strategies for typing previously untypeable strains.

@article{Simmonds2008NewOP,
  title={New oligonucleotide primers for P-typing of rotavirus strains: Strategies for typing previously untypeable strains.},
  author={Mirjam K{\"u}hne Simmonds and George Armah and Richard Asmah and Indrani Banerjee and Susan Damanka and Mathew Dioh Esona and Jon R. Gentsch and Jim J. Gray and Carl D Kirkwood and Nicola Page and Miren Iturriza-G{\'o}mara},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2008},
  volume={42 4},
  pages={368-73}
}
BACKGROUND The use of molecular methods for rotavirus characterisation provides increased sensitivity for typing, and allows the identification of putative reassortant strains. However, due to the constant accumulation of point mutations through genetic drift; and to the emergence of novel genotypes; and possibly zoonotic transmission and subsequent reassortment, the reagents and methods used for genotyping require close monitoring and updating. OBJECTIVES To design and evaluate a new VP4… CONTINUE READING