New multifunctional Escherichia coli-Streptomyces shuttle vectors allowing blue-white screening on XGal plates.

@article{Wehmeier1995NewME,
  title={New multifunctional Escherichia coli-Streptomyces shuttle vectors allowing blue-white screening on XGal plates.},
  author={Udo F. Wehmeier},
  journal={Gene},
  year={1995},
  volume={165 1},
  pages={
          149-50
        }
}
Four new shuttle vectors for Escherichia coli (Ec) and Streptomyces, pUWL218, pUWL219, pUWL-SK and pUWL-KS, which permit recognition of recombinant (re-) plasmids on XGal plates in Ec, were constructed. These vectors contain the replication functions of the Streptomyces wide-host-range multicopy plasmid pIJ101, the tsr gene conferring resistance to thiostrepton in Streptomyces, the ColEI origin of replication from the pUC plasmids for replication in Ec and the bla gene conferring resistance to… CONTINUE READING

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