New molecular diagnosis for pebrine inspection in silkworm eggs using a real-time PCR probe

@inproceedings{Rafeie2018NewMD,
  title={New molecular diagnosis for pebrine inspection in silkworm eggs using a real-time PCR probe},
  author={Farjad Rafeie and Mohammad Hosein Rezadoust and Ramin Abdoli},
  year={2018}
}
Abstract Pebrine is a most deadly disease of silkworm caused by infection with the microsporidium Nosema bombycis with extensive economic losses as a result of mortality in the end-stage of training. Several diagnosis methods have been established for detection of the disease, but most of these approaches are very old and rely on microscopic inspection and therefore are low in accuracy and speed. In the present study, a PCR-based method using a pair of specific primers only and a real-time PCR… CONTINUE READING