New model for the study of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane: the gelatin sponge/chorioallantoic membrane assay.

@article{Ribatti1997NewMF,
  title={New model for the study of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane: the gelatin sponge/chorioallantoic membrane assay.},
  author={Domenico Ribatti and Anna Gualandris and Maria Bastaki and Angelo Vacca and Monica Iurlaro and Luisa Roncali and Marco Presta},
  journal={Journal of vascular research},
  year={1997},
  volume={34 6},
  pages={455-63}
}
Several methods for the in vivo study of angiogenesis are available, and each angiogenic assay presents distinct advantages and disadvantages. In this study, we present a new method for the quantitation of angiogenesis and antiangiogenesis in the chick embryo chorioallantoic membrane (CAM), based on the implantation of gelatin sponges on the top of growing CAM, on day 8 of incubation. After implantation, the sponges were treated with a stimulator (recombinant human basic fibroblast growth… CONTINUE READING
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