New methods for the study of complex enzyme kinetics illustrated by analysis of the wavy curves of v versus (S) and non-linear double-reciprocal plots for human-placental 15-hydroxyprostaglandin dehydrogenase.

@article{Bardsley1976NewMF,
  title={New methods for the study of complex enzyme kinetics illustrated by analysis of the wavy curves of v versus (S) and non-linear double-reciprocal plots for human-placental 15-hydroxyprostaglandin dehydrogenase.},
  author={William G. Bardsley and M. James C. Crabbe},
  journal={European journal of biochemistry},
  year={1976},
  volume={68 2},
  pages={
          611-9
        }
}
A new method for discovering the minimum degree of rate equations using only the experimental graphs and a straight-edge (transparent ruler) is presented. This method is then illustrated by an analysis of the wavy v vs [S] curves and non-linear double-reciprocal plots reproducibly given by NAD-dependent 15-hydroxyprostaglandin dehydrogenase for which a new improved purification is described. It is shown by this analysis that the enzyme has a complex mechanism involving cooperatively linked… 
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