New methods for the primary culture of gill epithelia from freshwater rainbow trout

@article{Wood2004NewMF,
  title={New methods for the primary culture of gill epithelia from freshwater rainbow trout},
  author={Chris M Wood and Brighitta Eletti and Peter P{\"a}rt},
  journal={Fish Physiology and Biochemistry},
  year={2004},
  volume={26},
  pages={329-344}
}
Gill epithelia from freshwater rainbow trout can be grown in primary culture in Leibovitz's L-15 medium, by seeding freshly isolated gill cells on two successive days, from two different fish, directly onto permeable filter supports (DSI technique). This preparation allows the measurement of transepithelial resistance (TER) and exposure of the apical surface to freshwater, as in vivo. New culture methods were developed and evaluated, using TER as an indicator of epithelial integrity, in an… CONTINUE READING
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