New method to measure telomerase activity by transcription-mediated amplification and hybridization protection assay.

@article{Hirose1998NewMT,
  title={New method to measure telomerase activity by transcription-mediated amplification and hybridization protection assay.},
  author={Minoru Hirose and Junko Abe-Hashimoto and Hidetoshi Tahara and Toshinori Ide and Tadashi Yoshimura},
  journal={Clinical chemistry},
  year={1998},
  volume={44 12},
  pages={
          2446-52
        }
}
Telomerase is a ribonucleoprotein complex that uses RNA as a template for the addition of telomeric repeats. The development of the telomeric repeat amplification protocol (TRAP), a sensitive PCR-based assay, has facilitated the detection of telomerase activity in small tissue and tumor samples. Telomerase activity is expected to be a new diagnostic and prognostic marker of human cancer. In this study, we applied a non-PCR-based transcription-mediated amplification (TMA) and hybridization… CONTINUE READING

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