New method for the determination of the half inhibition concentration (IC50) of cholinesterase inhibitors.

@article{Kovov2013NewMF,
  title={New method for the determination of the half inhibition concentration (IC50) of cholinesterase inhibitors.},
  author={Mark{\'e}ta Kov{\'a}řov{\'a} and Karel Komers and {\vS}{\'a}rka {\vS}těp{\'a}nkov{\'a} and Patrik Par{\'i}k and Alexander Cegan},
  journal={Zeitschrift fur Naturforschung. C, Journal of biosciences},
  year={2013},
  volume={68 3-4},
  pages={133-8}
}
A new and simple analytical method is described for the determination of the IC50 values of the inhibitors of the hydrolysis of acetylcholine (ACh) or acetylthiocholine (ATCh) by cholinesterases. The method is based on monitoring the time course of the pH value during the uninhibited and inhibited reaction. It requires only a pH meter with a suitable pH measuring cell and a small thermostated stirred batch reactor. The method has been validated for twelve different types of cholinesterase… CONTINUE READING