New mammary epithelial and fibroblastic cell clones in coculture form structures competent to differentiate functionally

@article{Reichmann1989NewME,
  title={New mammary epithelial and fibroblastic cell clones in coculture form structures competent to differentiate functionally},
  author={Ernst Reichmann and Rick Ball and Bernd Groner and Robert Friis},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={108},
  pages={1127 - 1138}
}
We have established and characterized a spontaneously immortalized, nontumorigenic mouse mammary cell line, designated IM-2. IM-2 cells synthesize large amounts of the milk protein beta-casein upon addition of lactogenic hormones. The induction of beta-casein occurs rapidly and does not require any exogenous extracellular matrix components. The IM-2 cell line is morphologically heterogeneous and could be separated into cell clones with epithelial and fibroblastic characteristics. In monoculture… CONTINUE READING