New instruments for the laboratory diagnosis of hog cholera using the fluorescent antibody technique

Abstract

Acid p h o s p h a t a s e a c t i v i t y was d e t e r m i n e d in cu l tu res of Klebsiella grown in s e m i s y n t h e t i c med ia c o n t a i n i n g glucose a n d succ ina te as source of carbon . T he p h o s p h a tase s u b s t r a t e was 7.6 m M para-nitrophenyl-phosphate in a p H 4.9, 0.09 M c i t ra te buf fe r solut ion. Bac t e r i a l cu l tu res were d i lu ted ou t in buf fe r and a d j u s t e d w i t h m e a n s of an E E L n e p h e l o m e t e r to 58 % of t he dev i a t i on of t he ga lvanom e t e r (100% was t he g round glass s t anda rd ) . Af t e r m inu t e s c o n t a c t of a l iquo ts of suspens ion and r e a g e n t followed b y cen t r i fuga t ion , t he s u p e r n a t a n t was r ead in a Spec t ron ic 20 a t 405 nm. The d i s t r i b u t i o n of the va lues ob t a ined w i t h i n each of t h e 3 m a i n species of Klebsiella in th i s s t u d y exh ib i t ed a single peak. The m ed i ans were as follow: 14. species 0.3-0.4; K, aerogenes 0.2-0.3; K. oxytoca 0-0.2. The s t r a ins h a d been isola ted f rom clinical cases in t he u n i v e r s i t y hospi ta l , Cr i te r ia def in ing t he seve r i ty of cases were es tab l i shed . There was a n agreem e n t be tween p h o s p h a t a s e a c t i v i t y and seve r i t y of cases as if t he s t r a in s possess ing h igh p h o s p h a t a s e a c t i v i t y were more v i r u l e n t t h a n t he others . Comparison of the Sensit iv i ty of E l e c t r o i m m u n o diffusion, Reversed Pass ive Hemagglut inat ion , Latex Tes t and R a d i o i m m u n o a s s a y for Hepat i t i s B Antigen

DOI: 10.1007/BF01936790

Cite this paper

@article{Bommeli2005NewIF, title={New instruments for the laboratory diagnosis of hog cholera using the fluorescent antibody technique}, author={W. Bommeli}, journal={Experientia}, year={2005}, volume={33}, pages={131-131} }