New insight into the mechanism underlying fibroin secretion in silkworm, Bombyx mori.

  title={New insight into the mechanism underlying fibroin secretion in silkworm, Bombyx mori.},
  author={Dingpei Long and Weijian Lu and Yang Zhang and Qing Guo and Zhonghuai Xiang and Aichun Zhao},
  journal={The FEBS journal},
  volume={282 1},
In order to investigate the role of different parts of the fibroin heavy chain (H-chain) in the secretion of fibroin in the silk gland of the silkworm (Bombyx mori) in vivo, two enhanced green fluorescent protein (EGFP)/H-chain fusion genes with deduced protein sequences containing an identical N-terminal region and different C-terminal regions of the H-chain were introduced into the B. mori genome using a piggyBac-mediated germline transformation. EGFP fluorescence and molecular analysis… CONTINUE READING
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