New expression system tightly controlled by zinc availability in Lactococcus lactis.

@article{Llull2004NewES,
  title={New expression system tightly controlled by zinc availability in Lactococcus lactis.},
  author={Daniel Llull and Isabelle Poquet},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 9},
  pages={5398-406}
}
Here we developed the new expression system P(Zn) zitR, based on the regulatory signals (P(Zn) promoter and zitR repressor) of the Lactococcus lactis zit operon, involved in Zn(2+) high-affinity uptake and regulation. A P(Zn) zitR-controlled expression vector was constructed, and expression regulation was studied with two reporter genes, uspnuc and lacLM; these genes encode, respectively, a protein derived from Staphylococcus aureus secreted nuclease and Leuconostoc mesenteroides cytoplasmic… CONTINUE READING
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