New experimental approaches in retrovirus-mediated expression screening.

  title={New experimental approaches in retrovirus-mediated expression screening.},
  author={Toshio Kitamura},
  journal={International journal of hematology},
  volume={67 4},
We have established an expression screening system that utilizes retrovirus-mediated gene transfer. The system is based on a high-efficiency packaging cell line BOSC23 and retrovirus vectors pBabeX and pMX which are suitable for cDNA library construction. The combination of BOSC23 packaging cells and the pBabeX and pMX retrovirus vectors produces high-titer recombinant retroviruses, which are able to cover large complexities of cDNA libraries. These retroviruses gave 100% infection efficiency… CONTINUE READING

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