New epidermal keratin genes from Xenopus laevis: hormonal and regional regulation of their expression during anuran skin metamorphosis.

@article{Watanabe2001NewEK,
  title={New epidermal keratin genes from Xenopus laevis: hormonal and regional regulation of their expression during anuran skin metamorphosis.},
  author={Yusuke Watanabe and Hitoshi Kobayashi and Kunio Suzuki and Kaori Kotani and Katsutoshi Yoshizato},
  journal={Biochimica et biophysica acta},
  year={2001},
  volume={1517 3},
  pages={339-50}
}
Xenopus larval keratin (XLK) was isolated by gel electrophoresis of proteins of tadpole skin. Screening of an expression cDNA library of tail tissues by specific polyclonal antibodies against XLK produced XLK cDNA (xlk). Its complete nucleotide and predicted amino acid sequences revealed that XLK was a new member of type II keratin. Screening of a cDNA library of adult Xenopus skin using an oligonucleotide probe which had been designed from well-conserved N-terminal amino acid sequences of the… CONTINUE READING
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