New developments in the measurement of the hemagglutinin content of influenza virus vaccines by single-radial-immunodiffusion.

  title={New developments in the measurement of the hemagglutinin content of influenza virus vaccines by single-radial-immunodiffusion.},
  author={M. S. Williams and R. Mayner and N. J. Daniel and M. Phelan and S. Rastogi and F. M. Bozeman and F. Ennis},
  journal={Journal of biological standardization},
  volume={8 4},
A variety of detergents were compared as solubilizing agents for the measurement of the hemagglutinin (HA) content of influenza vaccines by the single-radial-immunodiffusion (SRID) assay. The surfactant, Emulphogene (BC-720), was found to be generally the most satisfactory of the detergents tested against a variety of vaccine strains. In particular, the zone size for B/Hong Kong and A/USSR/77 strains was found to be unaffected by a wide range of BC-720 concentrations (1–4%) in contrast to the… Expand
Mechanism of a decrease in potency for the recombinant influenza A virus hemagglutinin H3 antigen during storage.
Results demonstrate that the potency loss over time did not correlate with trends in changes to the higher order structure or hydrodynamic size of the rHA, and the most likely mechanism for the early loss of potency was disulfide-mediated cross-linking of rHA. Expand
Simultaneous quantification of the viral antigens hemagglutinin and neuraminidase in influenza vaccines by LC-MSE.
A method for vaccine analysis that yields simultaneous quantification of HA and NA levels much more rapidly than conventional HA quantification techniques, while providing additional valuable information on the total protein content is reported. Expand
The quantification of the haemagglutinin content of influenza whole virus and Tween-ether split vaccines.
It is concluded that precise quantification of Tween-ether split vaccines is not possible by the SRID test alone and as aggregate formation of solubilized haemagglutinin occurs, it is suggested that either a physico-chemical method including a disaggregation procedure, such as SDS treatment, or immunological evaluation of the original whole virus preparation before disruption of virions should be applied as an additional criterion for quantification. Expand
Single-radial-immunodiffusion as an in vitro potency assay for human inactivated viral vaccines.
Clinical studies demonstrate that standardization of influenza vaccines by SRID provides a better correlate of human immunogenicity than previous methods. Expand
Quantification of haemagglutinin of influenza Tween-ether split vaccines by immunodiffusion.
From the experiments, it is concluded that precise quantification of solubilized haemagglutinin is not achievable by the single radial immunodiffusion test alone and a physico-chemical method including an effective disaggregation procedure like SDS treatment in combination with PAGE is recommended. Expand
Assessment of influenza A neuraminidase (subtype N1) potency by ELISA.
Development of enzyme-linked immunosorbent assays (ELISAs) that are suitable for quantitation of the native form of NA of subtype N1 may expedite the development of NA-based influenza vaccines by providing a practical assay to measure NA potency. Expand
Evaluation of the single radial-immunodiffusion assay for measuring the glycoprotein content of rabies vaccines.
Modification of the single radial immunodiffusion technique and the feasibility of using this assay for the determination of rabies vaccine potency are discussed. Expand
Evaluation of standard reagents for radial-immunodiffusion assays. In vitro control of rabies vaccines.
The results of the validation study show that the INPPAZ (PAHO/WHO) is capable of producing and distributing the above-mentioned standard reagents, as well as of providing support for the incorporation of the RID technique (sensitive, rapid and inexpensive) to the laboratories that manufacture rabies vaccines in Latin America and the Caribbean. Expand
1. ABSTRACT The current influenza virus vaccines made of inactivated particles often induce undesirable local and general pyrogenic reactions particularly among youngsters. We have made a selectiveExpand
An alternative method for preparation of pandemic influenza strain-specific antibody for vaccine potency determination.
The results demonstrate a feasible approach for addressing one of the potential bottlenecks in inactivated pandemic influenza vaccine production and are particularly important in light of the difficulties in preparation of potency reagent antibody for pandemic H1N1 (2009) virus vaccines. Expand


Quantitation of influenza vaccine hemagglutinin by immunoelectrophoresis.
A technique for the quantitation of hemagglutinin (HA) in influenza vaccines by immunoelectrophoresis (IEP) gives accurate, and reproducible results and requires approximately 9 h to complete. Expand
An improved single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen: application for potency determinations of inactivated whole virus and subunit vaccines.
Findings support the conclusion that techniques based on the agglutination of erythrocytes may provide data on vaccine potency which are not directly comparable from strain to strain for ‘whole virus’ vaccines and that these methods are entirely inappropriate to potency assays of split-product or subunit vaccines. Expand
Crystalline antigen from the influenza virus envelope.
It was found that although the neuraminidase protein was completely degraded during proteolysis, the isolated haemagglutinin protein could be recovered almost unchanged from the incubation mixture and its preliminary chemical and antigenic characterization was reported. Expand
Correlation of laboratory studies with clinical responses to A/New Jersey influenza vaccines.
Large, uniformly performed clinical investigations with influenza A/New Jersey vaccines provided an opportunity to correlate results of laboratory tests of vaccine with human reactivity and antibody responses, and significant differences in immunogenicity and reactivity were observed in unprimed individuals. Expand
A surface antigen influenza vaccine. 1. Purification of haemagglutinin and neuraminidase proteins.
Influenza virus was centrifuged in a KII rotor through a sucrose gradient containing Triton N101, a non-ionic surfactant and the haemagglutinin and neuraminidase proteins were stripped from the surface and remained near the Surfactant micelles. Expand
Purification of neuraminidase from influenza viruses by affinity chromatography.
  • D. Bucher
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 1977
The neuraminidase (acylneuraminyl hydrolase, EC of the influenza virus recombinant strain (HON2) was solubilized with detergents and isolated by affinity chromatography and showed an increase in specific activity. Expand
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four majorExpand
O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.
  • J. R. Benson, P. E. Hare
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1975
O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products that can be detected easily and soluble and stable in aqueous buffers. Expand