New defective RNAs from citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation.

@article{Aylln1999NewDR,
  title={New defective RNAs from citrus tristeza virus: evidence for a replicase-driven template switching mechanism in their generation.},
  author={Mar{\'i}a A. Ayll{\'o}n and Carmelo L{\'o}pez and Jes{\'u}s Navas-Castillo and Munir Mawassi and William O. Dawson and Jos{\'e} Guerri and Ricardo Flores and Pedro Ochoa Moreno},
  journal={The Journal of general virology},
  year={1999},
  volume={80 ( Pt 3)},
  pages={817-21}
}
Defective RNAs (D-RNAs) ranging in size from 1968 to 2759 nt were detected in four citrus tristeza virus (CTV) isolates by hybridization of electroblotted dsRNAs with two probes specific for the 5'- and 3'-terminal genomic regions. The RNAs that hybridized with both probes were eluted, cloned and sequenced. Comparison with the sequences of the corresponding genomic regions of the helper virus showed, in all cases, over 99% nucleotide identity and direct repeats of 4-5 nt flanking or in the… CONTINUE READING