New deblocking aminopeptidases from Pyrococcus horikoshii.

@article{Mori2005NewDA,
  title={New deblocking aminopeptidases from Pyrococcus horikoshii.},
  author={Kazushige Mori and Kazuhiko Ishikawa},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2005},
  volume={69 10},
  pages={1854-60}
}
It has been reported that one of the hyperthermostable aminopeptidases from Pyrococcus horikoshii exhibits hydrolytic activity toward short peptides and acyl-peptides (deblocking activity). In the genome database of P. horikoshii, two new open reading frames homologous to the hyperthermostable aminopeptidase of P. horikoshii were found. The two new genes for the proteins were cloned, expressed using E. coli, and characterized. The purified proteins gave a single band on SDS-PAGE corresponding… CONTINUE READING