New cloning vectors for integration in the lambda attachment site attB of the Escherichia coli chromosome.

@article{Diederich1992NewCV,
  title={New cloning vectors for integration in the lambda attachment site attB of the Escherichia coli chromosome.},
  author={Ludger Diederich and Laura J Rasmussen and Walter Messer},
  journal={Plasmid},
  year={1992},
  volume={28 1},
  pages={14-24}
}
A set of plasmid cloning vectors has been constructed, allowing the integration of any DNA fragment into the bacteriophage lambda attachment site attB of the Escherichia coli chromosome. The system is based upon two components: (i) a number of cloning vectors containing the lambda attachment site attP and (ii) a helper plasmid, bearing the lambda int gene, transcribed from the lambda PR promoter under the control of the temperature-sensitive repressor cI857. The DNA fragment of interest is… CONTINUE READING