New cloning vectors and techniques for easy and rapid restriction mapping.

@article{Tartof1988NewCV,
  title={New cloning vectors and techniques for easy and rapid restriction mapping.},
  author={Kenneth D. Tartof and C A Hobbs},
  journal={Gene},
  year={1988},
  volume={67 2},
  pages={169-82}
}
We have modified plasmid, phage lambda and cosmid cloning vectors to be of general use for easily and unambiguously determining restriction maps of recombinant DNA molecules. Each vector is constructed so that it contains the rarely found NotI restriction site joined to a short synthetic linker sequence that is followed by a multiple cloning site. DNA cloned into these vectors may be restriction-mapped by either of two methods. In one technique, the cloned DNA is completely digested with NotI… CONTINUE READING
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