New cell biological applications of the laser microbeam technique: the microdissection and skinning of muscle fibers and the perforation and fusion of sarcolemma vesicles.

@article{Veigel1994NewCB,
  title={New cell biological applications of the laser microbeam technique: the microdissection and skinning of muscle fibers and the perforation and fusion of sarcolemma vesicles.},
  author={C Veigel and R W Steubing and Abdellah Harim and Cornelia Weber and Karl Otto Greulich and Rainer H. A. Fink},
  journal={European journal of cell biology},
  year={1994},
  volume={63 1},
  pages={140-8}
}
In a novel approach, the laser microbeam technique was used to selectively perforate the sarcolemma of skeletal muscle fibers, to prepare fragments of myofibrillar bundles of very small dimensions, and to induce fusion of sarcolemma vesicles. Using a highly focused UV laser microbeam with an effective beam diameter of down to 0.5 micron, very small (< 3 microns) myofibrillar fragments with an intact sarcomere striation pattern were obtained. When small amounts of Ca2+ were released in the… CONTINUE READING