New approach to the study of transient protein conformations: the formation of a semiburied salt link in the folding pathway of barnase.

Abstract

We use in this study a novel kinetic approach to determine the H+ titration properties of a semiburied salt link in the transition state for unfolding of barnase. The approach is based on changes in the pH dependence of the kinetics upon mutation of a target residue. This makes it relatively insensitive to the absolute value of the stability and, thereby… (More)

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Cite this paper

@article{Oliveberg1996NewAT, title={New approach to the study of transient protein conformations: the formation of a semiburied salt link in the folding pathway of barnase.}, author={Mikael Oliveberg and A. R. Fersht}, journal={Biochemistry}, year={1996}, volume={35 21}, pages={6795-805} }