New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm

@article{Zhao2009NewAH,
  title={New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm},
  author={Aichun Zhao and Tianfu Zhao and Yuansong Zhang and Qingyou Xia and Cheng Lu and Zeyang Zhou and Zhonghuai Xiang and Masao Nakagaki},
  journal={Transgenic Research},
  year={2009},
  volume={19},
  pages={29-44}
}
We constructed three different fibroin H-chain expression systems to estimate the efficacy of producing recombinant proteins in the cocoon of transgenic silkworms. The results showed that the three different EGFP/H-chain fusion genes were all expressed selectively in the posterior silk gland of the transgenic silkworm. The recombinant protein content of transgenic silkworm cocoons is up to 15% (w/w) when using the most highly efficient H-chain expression system. To our knowledge, in comparison… CONTINUE READING

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Molecule cloning: a laboratory manual, 2nd edn

  • J Sambrook, EF Fritsch, T Maniatis
  • 1989
Highly Influential
2 Excerpts

Improving cell-adhesive

  • T Tamura, T Asakura
  • 2007

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