New and better protocols for a short-term Caco-2 cell culture system.

@article{Yamashita2002NewAB,
  title={New and better protocols for a short-term Caco-2 cell culture system.},
  author={Shinji Yamashita and Keisuke Konishi and Yukako Yamazaki and Yoshir Taki and Toshiyasu Sakane and Hitoshi Sezaki and Yukihiro Furuyama},
  journal={Journal of pharmaceutical sciences},
  year={2002},
  volume={91 3},
  pages={
          669-79
        }
}
The aim of the present study was to develop new and better protocols for a short-term Caco-2 cell culture system for use in rapid screening of intestinal drug absorption. Caco-2 cells were cultured according to several protocols for short-term cell culture to obtain monolayers. The effects of serum (fetal bovine serum, FBS) in the culture medium and of the period of cell culture on the barrier function and transporter activities of the monolayers were examined. The barrier function was… CONTINUE READING
BETA

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 42 CITATIONS

Development, validation, and application of a novel 7-day Caco-2 cell culture system.

  • Journal of pharmacological and toxicological methods
  • 2014
VIEW 3 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED

Down-regulation of intestinal drug transporters in chronic renal failure in rats.

  • The Journal of pharmacology and experimental therapeutics
  • 2006
VIEW 3 EXCERPTS
CITES METHODS
HIGHLY INFLUENCED