New Reflected-Light Microscope for Viewing Unstained Brain and Ganglion Cells

  title={New Reflected-Light Microscope for Viewing Unstained Brain and Ganglion Cells},
  author={M D Egger and Mojm{\'i}r Petr{\'a}ň},
  pages={305 - 307}
We have designed and constructed a new type of reflected-light microscope to form images including only light reflected near the plane of the object. This selectivity of image formation is based on a mechanical flying-spot technique. Objects difficult or impossible to see with earlier microscopes, such as unstained cells and cell processes in brains of living salamanders and in excised dorsal root ganglia of frogs, have been observed routinely with this microscope. 
Foundations of Confocal Scanned Imaging in Light Microscopy
Seldom has the introduction of a new instrument generated as instant an excitement among biologists as the laser-scanning confocal microscope. With the new microscope, one can slice incredibly clean,
Scanning laser microscope for biological investigations.
The theory and design of a special purpose scanning laser microscope, particularly suited for biological investigations, is described, intended for the observation of objects embedded within transparent or translucent bodies, such as nerve cells in an intact brain.
Scanning Laser Microscope
A prototype of a scanning microscope for thick objects of low reflectivity and low optical contrast is designed and built and in a year or two it is expected to have a microscope suitable for advancing studies of brain cells in living animals.
A light efficient optically sectioning microscope
We describe a simple method by which optically sectioned images may be obtained. The system geometry is similar to that of a tandem scanning microscope but a one‐dimensional grid pattern is used
The tandem scanning reflected light microscope
Reflected-light microscopy of semitransparent material, such as unstained nervous tissue, is usually unsatisfactory because of low contrast and light scattering. In a new microscope both the object
Confocal Microscopy Of The Eye.
The ability to observe the microscopic world with our eye is limited. Early developments in microscopy involved improvements in lens design and construction (an understanding of the theory of
In Vivo Microscopy Using the Tandem Scanning Microscope a
The tandem scanning reflected light microscope (TSRLM) was invented to permit the examination of internal structure in live bulk tissue (e.g. brain, muscle, eye). Our TSRLM was the first of the
Tandem-scanning reflected-light microscope.
Reflected light microscopy of biological material has been a very difficult task and many different but hardly successful attempts have been made to get usable images. The main reasons for this state
Volume microscopy of biological specimens based on non-confocal imaging techniques
An approach that uses advanced computer graphics workstations and volume rendering algorithms for accurate reconstruction of volumetric microscopy data is described. It has been found that excellent


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Refereice%s aiid Notes