New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species.

@article{Castilho2003NewPA,
  title={New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species.},
  author={Tiago Moreno Castilho and Jeffrey Shaw and Lucile Maria Floeter-Winter},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 2},
  pages={
          540-6
        }
}
Glucose-6-phosphate dehydrogenase (G6PD) is one of the multilocus enzymes used to identify Leishmania by zymodeme analysis. The polymorphic pattern revealed by partial characterization of the gene encoding G6PD generated molecular markers useful in the identification of different Leishmania species by PCR. Initially degenerate oligonucleotides were designed on the basis of data on the conserved active center described for other organisms. Primers for reverse transcription-PCR experiments… CONTINUE READING

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