New Mechanisms of Flucytosine Resistance in C. glabrata Unveiled by a Chemogenomics Analysis in S. cerevisiae

@article{Costa2015NewMO,
  title={New Mechanisms of Flucytosine Resistance in C. glabrata Unveiled by a Chemogenomics Analysis in S. cerevisiae},
  author={Catarina Costa and Andreia Ponte and Pedro Pais and Rui Santos and Mafalda Cavalheiro and Takashi Yaguchi and Hiroji Chibana and Miguel C. Teixeira and Robert A. Arkowitz},
  journal={PLoS ONE},
  year={2015},
  volume={10}
}
5-Flucytosine is currently used as an antifungal drug in combination therapy, but fungal pathogens are rapidly able to develop resistance against this drug, compromising its therapeutic action. The understanding of the underlying resistance mechanisms is crucial to deal with this problem. In this work, the S. cerevisiae deletion mutant collection was screened for increased resistance to flucytosine. Through this chemogenomics analysis, 183 genes were found to confer resistance to this… Expand
Membrane Proteomics Analysis of the Candida glabrata Response to 5-Flucytosine: Unveiling the Role and Regulation of the Drug Efflux Transporters CgFlr1 and CgFlr2
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The response of the human pathogen Candida glabrata to 5-FC was evaluated at the membrane proteome level, using an iTRAQ-based approach, and the expression of both proteins, localized to the plasma membrane, was found to confer flucytosine resistance. Expand
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TLDR
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Identification of genetic markers of resistance to echinocandins, azoles and 5-fluorocytosine in Candida glabrata by next-generation sequencing: a feasibility study.
  • C. Biswas, S. Chen, +6 authors V. Sintchenko
  • Biology, Medicine
  • Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
  • 2017
TLDR
Genome-wide analysis of antifungal resistance markers was feasible and simultaneously revealed mutation patterns of genes implicated in resistance to different antIFungal drug classes. Expand
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